Electrophoresis Machine Essay Example for Free

Electrophoresis Machine Essay Gel electrophoresis is a laboratory procedure used to separate biological molecules with an electrical current. In this lesson, well review how agarose gel electrophoresis works and introduce the equipment necessary to perform an electrophoresis experiment. Separation of DNA molecules of different sizes can be achieved by using an agarose gel. Recall that agarose is a polysaccharide that can be used to form a gel to separate molecules based on size. Because of the gelatin-like nature of agarose, a solution of agarose can be heated and cooled to form a gel in a casting tray. Think of casting the agarose gel like pouring hot gelatin into a mold. The hot agarose liquid is poured into a casting tray. Once the mixture cools, a thin agarose brick will form. To ensure theres a place to put the DNA in the gel, a comb is placed in the agarose liquid before it cools. Each tooth in the comb will become a hole, or well, in the solidified agarose gel. Once cast, this gel is placed inside a piece of equipment called a gel box. An electrode one positive and one negative resides at each end of the gel box. The wells are always oriented, so theyre farther from the positive electrode. This ensures that the DNA molecules in the well must travel through the majority of the agarose gel, thus providing sufficient time for separation. Air isnt a great conductor of electricity, so we cover the gel with electrophoresis buffer. Electrophoresis buffer is a salt solution. It isnt table salt, but the salt ions can carry an electrical charge just like salt water can. The salt in the electrophoresis buffer completes the circuit between the positive and negative electrodes. When the electrodes of the gel box are connected to a power supply, electricity flows through the electrical circuit, causing the negatively charged DNA molecules to move into the agarose gel. The DNA molecules continue to travel through the agarose toward the positive electrode as long as an electrical current is present. Recall that shorter DNA molecules travel through agarose faster than longer DNA molecules. In this way, agarose gel electrophoresis separates different DNA fragments based on size. Once the samples are loaded, the electrical current supplied by the power supply not only moves the DNA samples through the gel but the dye molecules as well. Note the colored lines that appear. These lines do not represent the DNA fragments. These lines represent the dye in the loading buffer that was used to visualize the samples during the loading step. Once the gel run is complete, the agarose gel can be removed from the gel box and soaked in an ethidium bromide solution. Recall that ethidium bromide is used to visualize DNA. Ethidium bromide molecules intercalate, or insert, between the nitrogenous bases in a DNA molecule. In summary, gel electrophoresis is a laboratory procedure used to separate biological molecules with an electrical current. Together with a gel box and a power supply, an agarose gel can be used to separate DNA molecules based on size. Loading buffer enables scientists to insert DNA samples into the wells of the agarose gel. Once the electrophoresis procedure is initiated, the dye in the loading buffer forms a dye front that is used to determine when the procedure is complete. When the electrophoresis procedure is complete, the agarose gel can be soaked in an ethidium bromide solution to visualize the DNA bands on a UV box.

How do to Frames on Web Sites :: Essays Papers

How do to Frames on Web Sites Dividing a web page into frames is quite simple. Frames organize web pages by dividing them into rows or columns. The basic concept of frames is that each frame is a regular, complete html document. That means more than on one page is viewed in one single screen. This allows the viewers to stay one page other than having to keep on going to new pages. If a person wanted to divide a page into 2 side by side frames, he/she should put one complete html document in the left frame and another complete html document in the right frame. In order to make this happen, making an additional html document is needed. This third html document contains the *FRAME* tags that specify what goes where. That is actually its only function. The two tags used are the *FRAMESET* and the *FRAME* tags. A *FRAMESET* tag is a container tag, and a *FRAME* tag is not. For those who don't know what that means, a container tag has an opening *TAG* and a closing */TAG*. The *FRAME* tag specifies the html documents and whether the frame should have a border or a scrollbar for each html document. The *FRAMESET* tag does all the dividing. It specifies a few things regarding how to divide them up. (Note: If using web page providers such as angelfire and geocities, make sure the page that the frames code is going on is set for advanced html.) The most popular way frames are organized is by using two columns. One is simply being used for the menu of the web site, and the other being used as the main page to view a specific page within the web site. The examples used will include how to create frames showing the division of the page into two columns and also showing the division of the page into two rows. First of all, name the three html documents index.html, the page used for dividing it into frames; side1.html, the left column of the page used for the menu; and main.html, the page for viewing a specific page within the web site. Make sure the following html code is used in the index.html page: *FRAMESET cols="100,*†* *FRAME src=†side1.html†* *FRAME src=†main.html†* */FRAMESET*. The * simply means the space that is left over on the web page.

Response to George Orwell’s Shooting an Elephant

Although many people believe that racism has disappeared from our minds after all those solutions of trying to set equal rights in the world’s history, it still exists today, and will probably never vanish from our thinking. Some may clearly express it, whereas others may express racism unnoticeably, even to themselves. George Orwell, in â€Å"Shooting an Elephant†, tells a story of his past when he killed an elephant in order to please the Burmese crowd. At the time, Orwell was a white Indian Imperial Police officer who was disliked by the country's natives due to the fact that he was European. One day, he heard of an elephant's doing of ravaging the town, so he ran to the scene with a rifle. When he finally arrived, he found himself observing a peacefully feeding elephant while a huge crowd of Burmans gathered excitedly to observe his future action. Even though he does not feel it right to shoot the elephant, he has this huge, unavoidable pressure from the Burmese crowd. Eventually, his final decision was to shoot the elephant and satisfy the natives' hunger for excitement. Although the situation must have been difficult from Orwell's perspective, his action of killing the elephant cannot be justified. George Orwell's situation was definitely burdensome, looking from his perspective. He had his beliefs and feelings; yet, he vividly felt the pressure dawning on him as the natives assembled at the scene, eagerly and impatiently waiting for him to simply shoot the elephant. Especially for a person who â€Å"was all for the Burmese and all against their oppressors, the British,† he must have experienced an unbearable moment of making a decision. We all experience such burdensome pressure numerous times, starting from school as a kid. Students have such thing called peer pressure, where you are usually forced to do make a decision of whether or not to follow others' ways. Whether in academic studies, sports, or social life, they always tend to end up following others' beliefs and ways. For instance, peer pressure is the reason why the number of students who smoke since young age is rapidly increasing. However, conforming to others' beliefs and ways means that person is lacking self-confidence and self-respect. In order to survive this world at least a little more joyfully, people must follow their own beliefs and practices as many times as possible. Even though he had his belief about killing the elephant, Orwell purely abandoned the idea of following his way, and concentrated on how to please the natives. Only after he carried out the action did he admit that he â€Å"had done it solely to avoid looking a fool,† meaning he had no self-confidence or self-respect. In fact, by doing so what he decided to do, Orwell became a fool who was too afraid to speak out his belief: that the elephant does not deserve to be killed. As Orwell states, â€Å"he wears a mask, and his face grows to fit it. † Basically, people first hide their belief and conform to others’, then without knowing, they become to be one of them completely, losing their true selves. Following our own moral beliefs does not mean we must be ignorant of all outside beliefs. In other words, ignoring rules while living in a country with differing beliefs and laws is definitely a foolish act. For example, when I lived in Saudi Arabia, the national law forced us to wear black veils – called abbaya – like the local women outside home, and both the law and their belief forbid us to drink any kind of alcohol. Needless to say, we should not be fools as to go against any national laws to end up in prison, but our personal beliefs must still be kept safe. Furthermore, although Orwell faces a difficult situation, where he can either unnecessarily kill the elephant or decide not to, based on his own moral beliefs, he should have realized that he was the powerful one. Orwell explains his situation, â€Å"To come all that way, rifle in hand, with two thousand people marching at my heels, and then to trail feebly away, having done nothing – no, that was impossible. The crowd would laugh at me. And my whole life, every white man’s life in the East, was one long struggle not to be laughed at. But I did not want to shoot the elephant. † It was a situation in which he could not help but act according to the natives’ expectations. Nevertheless, readers must not forget that he was the one in control and power, with the rifle in his hands, and the natives could neither physically harm him nor report that he did the wrong thing. This meant that he really could have protected his moral values, and be ignorant of what others desperately wanted him to do since he believed the action to be wrong. The result of standing up to our own beliefs and practices can mean conflict with others who have different ones. But another consequence is faith in ourselves, self-confidence in our identity and ability to carry out those beliefs. Maintaining these will help us survive our lives more successfully, although probably not easily; on the other hand, if we continue to conform to others’ beliefs, we will come to depend only on others and have no self-respect. Overall, the action of killing the elephant by George Orwell is not justified because, despite his moral belief about not killing the elephant, he went straight against it and shot the elephant, winning the natives’ expectations, and overcoming the boundary between them, but losing his true self and his beliefs.

Traditional Utilitarianism. According To Our Text,...

Traditional Utilitarianism According to our text, utilitarianism is a general term for â€Å"any view that holds that actions and policies should be evaluated based on the benefits and costs they will impose on society† (Velasquez, 2012). In any circumstance, the right activity or approach is the one that will create the best net advantages or the most reduced net costs (when all options have just net expenses). Furthermore, utilitarianism rule holds that any activity is morally right if and just if the aggregate results of a similar demonstration are more than the results created by some other activity a specialist could have done in its place. As indicated by utilitarianism just a single activity is ideal in the last examination: the†¦show more content†¦In any case, customary utilitarians would preclude that an activity from securing a specific kind is dependably either right or off-base. Rather, each activity would need to be measured given its specific condition s. Utilitarian perspectives have additionally been exceedingly powerful in financial aspects. A long line of business analysts, starting in the nineteenth century, contended that monetary conduct could be clarified by expecting that individuals dependably endeavor to amplify their utility and that the utilities of products can be measured by the costs individuals will pay for them. Ecological Ethic Ecological ethics is the moral view that nonhumans parts of the earth should be saved for their own purpose, paying little mind to whether this gives individuals advantages. Additionally, we people have an obligation to not hurt them without adequately genuine reasons. There are a few assortments of biological morals, some more radical and expansive than others. The most famous form asserts that, notwithstanding individuals, different creatures have natural esteem and are meriting our regard and security. A few utilitarians, for example, Peter Singer, for instance have claimed that agony is a shrewdness whether it is incurred on people or on individuals from different species. Moreover, Singer contends that the torment experienced by aShow MoreRelatedEuthanasia Is The Act Of Killing Someone Painlessly1534 Words   |  7 Pagesparticularly utilitarianism and the Divine Command Theory, offer two perspectives on euthanasia in the realm of ethics and morality that allow us to understand that although euthanasia is never the preferred option, it should be accessible and permissible. Utilitarianism, as propounded by Jeremy Bentham, is concerned with one ultimate moral â€Å"principle of utility†. This moral principle holds that â€Å"we should always try to produce the greatest possible benefit for everyone who will be affected by our action†Read MoreAbortion (Marry Anne Warren â€Å"on the Moral and Legal Status of Abortion, † and â€Å"Utilitarianism.†)1611 Words   |  7 Pagesor non induced abortions are commonly known as miscarriages. Abortion has had a long and passionate history. It has been induced through various methods ranging from, herbal medicines, and the use of sharpened tools, physical trauma, and other traditional methods. The different legal and cultural views on abortion differ around the world, and in many regions of the world public debate over the ethics and legal ramifications of abortion are intense. The topic of abortion has brought about a seriesRead MoreCitigroup and Subprime Lending Case Study3619 Words à ‚  |  15 Pages714 -716 1. Are there moral concerns associated with subprime lending? Are those moral concerns based on utilitarianism, rights, or justice considerations? Before we discuss the first question let’s get a working description of what subprime leaning is. A subprime lender is financial entity that has an inclination to lend to consumers that are not qualified for traditional loans due to their poor credit status and history of repayment difficulties. Lending to subprime candidates helpedRead MoreThe Different Ways Of Which Freedom Can Be Compatible With Determinism1334 Words   |  6 Pages FINAL EXAM 1-Discuss the different ways in which freedom can be compatible with determinism. Answer: According to (Consider Ethics text book on page 224), Freedom and determinism can be compatible with Simple Compatibilism, which according to David Hume, he claimed that ‘’all the puzzles and disputes about free will result from sloppy and confused use of language. 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Decide whether PharmaCARE’s use of Colberian intellectual property would be ethical in accordance with: Utilitarianism, Deontology, Virtue ethics, Ethics of care, and my own moral / ethical compass. Analyze the way PharmaCARE uses U.S. law to protect its own intellectual property while co-opting intellectual property in Colberia. Suggest at least three ways theRead MoreStakeholder Analysis Essay1335 Words   |  6 PagesGroup and other private prison companies.† There is no law violation in Private Prison Corporation because they make a contract with government, federal agency who present the law. Another, Private Prison Corporation is not committing fraud. According the text, there is no sign of fraud in Corporation. They make money by signing contracts with state and federal governments to house-convicted criminal. Nevertheless, Private Prison Corporation is violating moral custom because their net income is increasingRead MoreEssay on Globalization of McdonaldS on China5339 Words   |  22 PagesMcDonald’s have encountered a few challenges that have hindered their progress. Globalization in China has proven to be one of the most difficult challenges. In China McDonald’s is a symbol of westernized way of living. The Chinese people are very traditional and loyal to its heritage. Many in China see McDonald’s as an invasion of their culture. McDonalds in China must make an effort to blend in with the culture and not seem like the great westernization invasion. Through a more diversified menuRead MoreUniversal Healthcare System in the United States2430 Words   |  10 PagesI will argue for a universal healthcare system in the United States through a comparison of feminist ethics and traditional ethics. I find that the following considerations from Virginia Held’s Ethics of Care, and Kant’s view on autonomy provide a convincing argument for universal healthcare coverage. Held In Virginia Held’s Ethics of Care, she makes six distinct statements on care ethics through a feminist point of view. 1.) â€Å"Moralities built on the image of the independent, autonomous,Read MoreThe Between Humans And Animals1461 Words   |  6 Pagesecosystems (Why Sport Hunting Is Cruel and Unnecessary, n.d.). Overall, this â€Å"exotic† pastime is opposed by the majority in the United States, resulting in the derivation of anti-hunting organizations and even the â€Å"No Hunting† signs often posted in even our own backyards. The opinions are subject to change depending on the motives of the individual hunter and the different uses of the sport. The relationship between humans and animals has greatly evolved within the past 12,000 years. With this being said